Fluorescence Microscopy

Main content

Our widefield and structured illumination microscope is typically used for live cell imaging or fast acquisition of 3D samples.

Table Fluorescence Microscope  
Table for the Fluorescence Microscopy

* The apotome only works through 170um glass coverslips, also called #1.5. It cannot work through plastic and is not calibrated for other coverslip thicknesses.

** The 25x objective has a ring to correct for oil and glycerol as well, but the default position is water. If you change it, please put it back.

*** In bold: works for sure (our objective is compatible according to its datasheet and has the appropriate prisms/rings on both sides).

In italic: objective compatible cf datasheet, but we don’t have the prisms (We don’t have the DIC0 and DIC2 prisms mounted on the microscope now).

**** Numerical aperture. Ideally, resolution=a*l/NA, with a between 0.47 and 1 depending on the definition of resolution used.

Filter Cubes

1- Transmitted light

2- GFP

3- DsRed

4- mRFP


Others extensions

Incubation chamber (temperature and CO2), autofocus, robotic stage (tile scans, multi-position time lapses…)

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Wed Jun 28 21:22:14 CEST 2017
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